Continuous photosynthetic biohydrogen production from acetate-rich wastewater: Influence of light intensity

Photo-biohydrogen by microalgae is attractive sustainable energy caused the utilization of solar and water. However, due to oxygen (O 2 ) sensitive hydrogenase (HydA) activity, effective control O light intensity critical for achieving photosynthetic hydrogen (H production. Here we demonstrate continuous algal H production using acetate-enriched fermenter effluent, complete cessation without sulfur depletion. Average 108 ± 4 μmol L −1 Chlamydomonas reinhardtii 88 7 Chlorella sorokiniana at 100 m −2 s were observed 15 days, respectively. The highest conversion efficiency (LHCE) 1.61% C. 1.06% Sorokiniana was obtained under low (50 but LHCE decreased with increase followed photoinhibition, which led a decrease HydA activity Low 50 LHCE, in exhibited photoinhibition biomass growth kinetics produce chlorophyll (Chl electron generation. These results that feasible strategy producing anoxygenic photosynthesis. • impact on during photolysis studied. maximum light-energy-to-hydrogen-energy reinhardtii. high intensities cause decreasing Continuous possible acetate-rich effluent.